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	Provedor de dados BJID (2) Autor Alonio,Virginia (1) Durand,Karina (1) Fellner,María Dolores (1) Ganjali,Zohre (1) Irazu,Lucía (1) Niazi,Abbas Ali (1) Picconi,María Alejandra (1) Ramroodi,Nourollah (1) Rodriguez,Marcelo (1) Sanadgol,Nima (1) Sarabandi,Vida (1) Mais... Palavra-chave EBV (2) ELISA (1) MS (1) PTLD (1) QPCR (1) Reactivation (1) Real-time PCR (1) Viral load (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2013 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Duplex realtime PCR method for Epstein-Barr virus and human DNA quantification: its application for post-transplant lymphoproliferative disorders detection BJID Fellner,María Dolores; Durand,Karina; Rodriguez,Marcelo; Irazu,Lucía; Alonio,Virginia; Picconi,María Alejandra. INTRODUCTION: The quantification of circulating Epstein-Barr virus (EBV) DNA is used to monitor transplant patients as an early marker of Post-Transplant Lymphoproliferative Disorders (PTLD). So far no standardized methodology exists for such determination.OBJECTIVE: Our purpose was to develop and validate a real-time PCR assay to quantify EBV DNA in clinical samples from transplant recipients.METHODS: A duplex real-time PCR method was developed to amplify DNA from EBV and from a human gene. The EBV load was determined in peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal tissue from 64 non-transplanted patients with lymphoid-hypertrophy (Non-Tx), 47 transplant recipients without PTLD (Tx), 54 recipients with PTLD (Tx-PTLD), and 66 blood... Tipo: Info:eu-repo/semantics/article Palavras-chave: EBV; Real-time PCR; Viral load; PTLD. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300271 Evaluation of reactive Epstein-Barr virus (EBV) in Iranian patient with different subtypes of multiple sclerosis (MS) BJID Ramroodi,Nourollah; Niazi,Abbas Ali; Sanadgol,Nima; Ganjali,Zohre; Sarabandi,Vida. OBJECTIVES: Epstein-Barr virus has been recently associated with the onset of multiple sclerosis, yet understanding how it elicits autoimmunity remains elusive. We investigated the relation between Epstein-Barr virus reactivation and disease development in different subtypes of multiple sclerosis. METHODS: In the present research, we have determined the Epstein-Barr virus-DNA load by quantitative real-time polymerase chain reaction and Epstein-Barr virus antibody levels by EIA technique in both multiple sclerosis patients (n = 78) and healthy controls (n = 123). RESULTS: Our results demonstrated increased titer of both anti-Epstein-Barr virus-IgG and IgM antibodies in patients (91.02% vs 82.11% in controls, p < 0.001 and 14.1% vs 4.06% in controls, p... Tipo: Info:eu-repo/semantics/article Palavras-chave: EBV; MS; Reactivation; QPCR; ELISA. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200007 Registros recuperados: 2 Primeira ... 1 ... Última

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